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Related Experiment Videos

Anomalies in sedimentation. V. Chains at high fields, practical consequences

B H Zimm, V N Schumaker, C B Zimm

    Biophysical Chemistry
    |July 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

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    This study presents a formula to predict how a chain molecule's sedimentation coefficient changes with centrifugal force. For DNA, this indicates a maximum sedimentation coefficient at a specific molecular weight, impacting band profiles.

    Area of Science:

    • Biophysics
    • Physical Chemistry
    • Molecular Biology

    Background:

    • Sedimentation analysis is crucial for determining molecular properties.
    • Previous theoretical work by Zimm (1974) provides a foundation for understanding non-ideal sedimentation behavior.
    • The influence of centrifugal field on sedimentation coefficients for chain molecules requires further quantitative analysis.

    Purpose of the Study:

    • To develop a predictive model for the change in sedimentation coefficient with increasing centrifugal field for chain molecules.
    • To derive a simple formula accurately describing this relationship.
    • To apply the findings to DNA and assess the implications for sedimentation analysis.

    Main Methods:

    • Utilized theoretical framework from a preceding paper by B.H. Zimm.

    Related Experiment Videos

  • Calculated a numerical table for sedimentation coefficient changes across varying centrifugal fields.
  • Derived and validated a fitting formula: S/So=(1+0.1155y^2)^-1/4.

    • Main Results:

    • A simple formula accurately predicts sedimentation coefficient changes within 1.3% up to a specific centrifugal field.
    • The formula S/So=(1+0.1155y^2)^-1/4 was established, where y relates molecular weight, initial sedimentation coefficient, and centripetal acceleration.
    • Application to DNA suggests a maximum sedimentation coefficient at a particular molecular weight, decreasing for higher molecular weights.

    Conclusions:

    • The derived formula provides a practical tool for analyzing sedimentation behavior of chain molecules.
    • For DNA, sedimentation velocity is not monotonically increasing with molecular weight at a given field strength.
    • The presence of a sedimentation coefficient maximum can significantly distort the profiles of sedimenting non-homogeneous bands.