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Related Experiment Videos

Mice carrying a CD20 gene disruption

T L O'Keefe1, G T Williams, S L Davies

  • 1Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.

Immunogenetics
|June 20, 1998
PubMed
Summary

Mice lacking the CD20 gene show no major changes in B lymphocyte function. However, a specific B cell population depletion was linked to genetic background, not CD20 disruption.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • CD20 is a B lymphocyte-specific antigen with an unknown function.
  • Proposed roles for CD20 include B cell activation, proliferation, and calcium transport.
  • A mouse homologue of CD20 exists with B cell-specific expression.

Purpose of the Study:

  • To investigate the function of CD20 by generating and analyzing mice with a CD20 gene disruption.
  • To assess the impact of CD20 deficiency on B lymphocyte differentiation and function.
  • To identify potential roles of CD20 in B cell signaling and calcium homeostasis.

Main Methods:

  • Generation of CD20 gene-disrupted mice through gene targeting.
  • Analysis of B lymphocyte populations using surface marker expression (e.g., B220).
  • Assessment of B cell function including signaling, proliferation, and calcium uptake.

Main Results:

  • CD20 gene disruption did not significantly affect B cell differentiation or function.
  • A substantial depletion of a specific peritoneal B cell subset (IgM+ B220-negative) was observed in homozygous knockout mice.
  • This depletion was found to be associated with the genetic background (129 vs. C57BL/6) and linked to the CD20 locus, not the gene disruption itself.

Conclusions:

  • The function of CD20 in B lymphocyte differentiation and activation remains largely undetermined based on this study.
  • Genetic background effects can significantly confound phenotype analysis in genetically engineered mouse models.
  • Caution is advised when interpreting results from mixed genetic background mouse crosses, particularly near linked loci like CD5.

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