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Related Experiment Videos

A role for vesicles in human basophil secretion

A M Dvorak1

  • 1Department of Pathology, East Campus, Beth Israel Deaconess Medical Center and Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA.

Cell and Tissue Research
|June 23, 1998
PubMed
Summary

Human basophils utilize vesicular transport for secretion, a process distinct from classical degranulation. This mechanism involves piecemeal degranulation and the budding of vesicles, facilitating the release of histamine and other proteins.

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Area of Science:

  • Immunology
  • Cell Biology
  • Histology

Background:

  • Human basophils are rare granulocytes involved in allergic responses.
  • Secretion mechanisms in basophils were not fully understood, particularly regarding vesicular transport.
  • Piecemeal degranulation, a novel secretion method, was observed in basophils.

Purpose of the Study:

  • To review evidence for vesicular transport in human basophil secretion.
  • To elucidate the mechanisms of piecemeal degranulation.
  • To establish the role of vesicles in releasing specific basophil products.

Main Methods:

  • Electron microscopy of skin lesions.
  • Isolation and purification of circulating human basophils.
  • Development of growth factors for basophil expansion.

Related Experiment Videos

  • Analysis of secretogogue mechanisms and secreted products.
  • Advanced ultrastructural preparations for imaging vesicles.

    • Main Results:

    • Piecemeal degranulation involves slow granule content release with empty granule retention.
    • Budding of vesicles from and to secretory granules was observed.
    • Vesicles were identified as a key mechanism for secreting histamine and Charcot-Leyden crystal protein.
    • A generalized degranulation model was proposed.

    Conclusions:

    • Vesicular transport is a significant mechanism for human basophil secretion.
    • Piecemeal degranulation is a unique pathway for releasing granule contents.
    • The developed tools and models enabled a deeper understanding of basophil secretion.