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Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region

H Ueda1, K Tsumoto, K Kubota

  • 1Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan. hueda@bio.t.u-tokyo.ac.jp

Nature Biotechnology
|December 1, 1996
PubMed

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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.