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Soluble guanylate cyclase in Saccharomyces cerevisiae
W N Kuo1, R N Kanadia, M Mcnabb
1Division of Science and Math, Bethune-Cookman College, Daytona Beach, Florida 32115, USA.
Biochemistry and Molecular Biology International
|June 23, 1998
Summary
Researchers found soluble guanylate cyclase (SGC) in yeast, suggesting nitric oxide/cyclic GMP signaling is important in unicellular eukaryotes. This NO/cyclic GMP pathway may be utilized both inside and outside yeast cells.
Area of Science:
- Biochemistry
- Molecular Biology
- Yeast Genetics
Background:
- Nitric oxide (NO) signaling pathways are crucial in many organisms.
- The role of NO/cyclic GMP signaling in unicellular eukaryotes like yeast is not well understood.
Purpose of the Study:
- To investigate the presence and function of soluble guanylate cyclase (SGC) in Saccharomyces cerevisiae.
- To explore the NO/cyclic GMP signaling pathway in yeast.
Main Methods:
- Immunoaffinity chromatography to purify yeast SGC.
- Western blot to detect SGC.
- ELISA to measure cyclic GMP levels and SGC activity.
- Incubation of yeast cells with NO donors and inhibitors.
Main Results:
- A 40kDa immunoreactive band of SGC was detected in yeast extract.
- Yeast SGC activity was modulated by NO donors (sodium nitroprusside, s-nitrosoglutathione) and an inhibitor (1H-(1,2,4)oxadiazolo(4,3-A)quinoxalin-1-one).
- Increased cyclic GMP levels were observed in yeast cells treated with an NO donor.
Conclusions:
- Soluble guanylate cyclase (SGC) is present in Saccharomyces cerevisiae.
- The NO/cyclic GMP signaling pathway is functional in yeast, operating both intracellularly and extracellularly.
- This finding highlights the potential significance of NO/cyclic GMP signaling in unicellular eukaryotes.