Antigen-induced responses in lung parenchymal strips during sinusoidal oscillation

We have recently demonstrated that tissue resistance increases during the early response to antigen challenge in sensitized Brown-Norway rats. The purpose of the present study was to investigate in vitro the dynamic tissue response to antigen challenge and the involvement of the mediators, i.e., serotonin (5HT) and leukotriene D4 (LTD4). In addition, we questioned whether strips from sensitized rats responded differently to nonspecific challenge compared with those of unsensitized controls. We sensitized Brown-Norway rats with ovalbumin and performed experiments using strips of subpleural parenchyma. Tissue strips were challenged with ovalbumin in the bath; in some experiments the tissues were exposed to methysergide (10(-6) M), a 5HT antagonist, or MK-571 (10(-6) M), a LTD4 receptor antagonist, or both, prior to challenge. At the end of the experiment all tissues were exposed to carbacholine (10(-3) M). Oscillation mechanics of tissue strips were studied and values of resistance (R), elastance (E), and hysteresivity (eta) were obtained. During ovalbumin challenge in sensitized tissues, R, E, and eta increased significantly (% change in R, 12.1 +/- 2.1%; % change in E, 3.8 +/- 1.3%; % change in eta, 9.3 +/- 2.8%). Both methysergide and MK-571 reduced the increase in R (3.0 +/- 0.6 and 3.2 +/- 0.8%, respectively, p < 0.05 vs. ovalbumin). There was no difference between sensitized and unsensitized strips in the response to carbacholine challenge. These data suggest that the oscillatory behaviour of tissue strips from sensitized rats is altered after ovalbumin challenge. The mechanism of that response is mediated by both 5HT and LTD4.