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Ixodes scapularis: salivary kininase activity is a metallo dipeptidyl carboxypeptidase
1Section of Medical Entomology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. JRibeiro@atlas.niaid.nih.gov
Abstract:
Saliva and salivary gland homogenates of Ixodes scapularis contain a dipeptidyl carboxypeptidase activity that accounts for the previously described salivary kininase activity of this tick. Reversed phase HPLC and laser desorption mass spectrography of the reaction products identified bradykinin fragment 1-7 and 1-5 as being produced subsequent to incubation of purified salivary kininase with bradykinin. The activity was inhibited by captopril and EDTA and was activated by cobalt and manganese, a behavior similar to that displayed by angiotensin-converting enzymes of vertebrate and invertebrate origins.