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Related Experiment Videos

Telomerase activity in 144 brain tumours

T Sano1, A Asai, K Mishima

  • 1Department of Neurosurgery, Faculty of Medicine, University of Tokyo, Japan.

British Journal of Cancer
|July 4, 1998
PubMed
Summary

Telomerase activity, crucial for cell proliferation, is significantly higher in malignant brain tumors compared to benign ones. This finding highlights telomerase as a potential biomarker for distinguishing cancerous brain lesions.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Unlimited cell proliferation in immortalized cells is linked to telomerase activity.
  • Telomerase synthesizes telomeric repeats at chromosome ends, maintaining cell viability.

Purpose of the Study:

  • To analyze and quantify telomerase activity in benign and malignant brain tumors.
  • To determine the association between telomerase activity and brain tumor malignancy.

Main Methods:

  • Utilized the polymerase chain reaction-based telomeric repeat amplification protocol (TRAP) assay.
  • Analyzed telomerase activity in 99 benign and 45 malignant brain tumors.
  • Quantified results by normalizing to the T98G glioma cell line and visual assessment.

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Main Results:

  • Malignant brain tumors showed a significantly higher positive rate of telomerase activity (57.8%) than benign tumors (2.0%).
  • Positive telomerase activity was quantified as 36.1% in malignant tumors versus 0% in benign tumors (P < 0.0001).
  • Quantification confirmed visual assessment, establishing a strong correlation between telomerase activity and malignancy.

Conclusions:

  • Positive telomerase activity is strongly associated with malignant brain tumors.
  • Telomerase activity is rare in benign brain tumors like neurinomas and meningiomas.
  • Telomerase serves as a potential diagnostic marker for brain tumor malignancy.