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Related Experiment Videos

SCL specifies hematopoietic mesoderm in Xenopus embryos

P E Mead1, C M Kelley, P S Hahn

  • 1Division of Hematology/Oncology, Howard Hughes Medical Institute, Children's Hospital and Dana-Farber Cancer Institute, Boston, MA, USA.

Development (Cambridge, England)
|June 24, 1998
PubMed
Summary

Stem cell leukemia (SCL) is essential for hematopoietic progenitor development. In Xenopus, SCL specifies mesoderm to a hematopoietic fate, similar to MyoD

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Area of Science:

  • Developmental biology
  • Hematopoiesis
  • Molecular genetics

Background:

  • Transcription factors are crucial for hematopoietic progenitor development.
  • Disruption of the SCL gene blocks early hematopoietic programs and affects all lineages.

Purpose of the Study:

  • To investigate the function of SCL in the organogenesis of blood during Xenopus embryogenesis.
  • To understand the role of SCL in specifying hematopoietic cell fate.

Main Methods:

  • Isolation of Xenopus SCL cDNAs.
  • Analysis of SCL expression patterns during embryogenesis.
  • Functional studies using explant assays and dominant-negative receptor experiments.

Main Results:

  • SCL is expressed in ventral mesoderm and its expression is induced by BMP-4.

Related Experiment Videos

  • SCL expression in explants induces globin protein expression.
  • Overexpression of SCL does not affect dorsal-ventral patterning.

    • Conclusions:

    • SCL is necessary and sufficient to specify hematopoietic mesoderm.
    • SCL plays a key role in specifying hematopoietic cell fate, analogous to MyoD's role in muscle cell fate.