Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Estimating sequestered parasite population dynamics in cerebral malaria

M B Gravenor1, M B van Hensbroek, D Kwiatkowski

  • 1Department of Pediatrics, Institute of Molecular Medicine, University of Oxford, Oxford, OX3 9DS, United Kingdom. michael.gravenor@paediatrics.oxford.ac.uk

Proceedings of the National Academy of Sciences of the United States of America
|June 24, 1998
PubMed
Summary

Estimating sequestered malaria parasites in tissues is crucial for understanding severe disease. A new mathematical model reveals sequestered parasite mass, not peripheral parasitemia, predicts fatal outcomes in cerebral malaria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caregivers and community perceptions of blood transfusion for children with severe anaemia in Uganda.

Transfusion medicine (Oxford, England)·2019
Same author

Variability in distribution and use of tuberculosis diagnostic tests in Kenya: a cross-sectional survey.

BMC infectious diseases·2018
Same author

Environmental noise spectroscopy with qubits subjected to dynamical decoupling.

Journal of physics. Condensed matter : an Institute of Physics journal·2017
Same author

A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children.

Genes and immunity·2015
Same author

A high-dose preparation of lactobacilli and bifidobacteria in the prevention of antibiotic-associated and Clostridium difficile diarrhoea in older people admitted to hospital: a multicentre, randomised, double-blind, placebo-controlled, parallel arm trial (PLACIDE).

Health technology assessment (Winchester, England)·2013
Same author

The 5q31 region in two African populations as a facet of natural selection by infectious diseases.

Genetika·2013

Area of Science:

  • * Malariology and Parasitology
  • * Mathematical Modeling in Infectious Diseases
  • * Clinical Tropical Medicine

Background:

  • * Assessing the burden of sequestered malaria parasites in tissues is critical for understanding severe malaria pathogenesis, including cerebral malaria.
  • * Current clinical investigations are limited by the absence of reliable methods to quantify these hidden parasite populations.

Purpose of the Study:

  • * To develop and validate a novel method for estimating the population of sequestered malaria parasites.
  • * To correlate the estimated sequestered parasite mass with clinical outcomes in children with cerebral malaria.
  • * To compare the efficacy of artemether and quinine in clearing both peripheral and sequestered parasites.

Main Methods:

  • * Development of an age-structured mathematical model of the malaria parasite erythrocyte cycle.

Related Experiment Videos

  • * Integration of clinical observations of peripheral parasitemia with the mathematical model.
  • * Application of the model to clinical data from 217 Gambian children treated for cerebral malaria.

    • Main Results:

    • * The mathematical model successfully estimated the population of sequestered malaria parasites.
    • * Artemether demonstrated faster clearance of peripheral parasitemia compared to quinine.
    • * Clearance rates for sequestered parasites were similar between artemether and quinine treatments.
    • * Estimated sequestered parasite mass was a more direct predictor of fatal outcome than peripheral parasitemia.

    Conclusions:

    • * The developed method enables sequential analysis of sequestered parasite dynamics in cerebral malaria patients.
    • * The findings suggest that the similar clearance of sequestered parasites may explain the comparable mortality reduction between artemether and quinine, despite artemether's faster effect on circulating parasites.
    • * Quantifying sequestered parasites offers a more accurate prognostic indicator for severe malaria outcomes.