Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cdc42 is required for membrane dependent actin polymerization in vitro

V Moreau1, M Way

  • 1Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.

FEBS Letters
|June 24, 1998
PubMed
Summary

Researchers developed an in vitro assay for membrane-dependent actin polymerization. They found Cdc42, a Rho family GTPase, is crucial for this process, unlike Listeria-stimulated actin assembly.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defective membrane repair machinery impairs survival of invasive cancer cells.

Scientific reports·2020
Same author

Baseline parameters for rotational thromboelastometry in healthy labouring women: a prospective observational study.

BJOG : an international journal of obstetrics and gynaecology·2020
Same author

Skin cancer multiplicity in lung transplant recipients: a prospective population-based study.

The British journal of dermatology·2019
Same author

Baseline parameters for rotational thromboelastometry (ROTEM®) in healthy pregnant Australian women: a comparison of labouring and non-labouring women at term.

International journal of obstetric anesthesia·2019
Same author

Identification and Quantification of Polymyxa graminis f. sp. temperata and P. graminis f. sp. tepida on Barley and Wheat.

Plant disease·2019
Same author

Widespread Occurrence of Wheat spindle streak mosaic virus in Belgium.

Plant disease·2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • In vitro actin-based motility assays using bacterial pathogens are vital for understanding actin dynamics and cell motility.
  • Existing systems primarily focus on pathogen-stimulated actin assembly, with less focus on membrane-initiated polymerization.

Purpose of the Study:

  • To develop a novel in vitro assay for studying membrane-dependent actin polymerization.
  • To investigate the role of small GTPases in membrane-initiated actin polymerization.

Main Methods:

  • Utilized endogenous membrane vesicles within Xenopus extracts.
  • Employed depletion and reconstitution experiments to identify key molecular players.
  • Focused on Rho family GTPases, including Cdc42, Rac, and Rho.

Related Experiment Videos

Main Results:

  • Established an in vitro assay for membrane-dependent actin polymerization.
  • Demonstrated that membrane-dependent actin polymerization requires small GTPases of the Rho family.
  • Identified Cdc42, but not Rac or Rho, as essential for stimulating actin polymerization from membranes.

Conclusions:

  • The developed in vitro system effectively models membrane-dependent actin polymerization.
  • Cdc42 is a key regulator of membrane-initiated actin polymerization, distinct from Listeria-induced pathways.
  • This assay system facilitates the identification of downstream Cdc42 effectors involved in membrane-associated actin dynamics.