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The HIS4 gene from the yeast Kluyveromyces lactis
M A Freire-Picos1, M Hampsey, M E Cerdán
1Departamento de Biologia Celular y Molecular, Facultad de Ciencias, Universidad de La Coruña, Spain.
Yeast (Chichester, England)
|June 25, 1998
Abstract:
The Kluyveromyces lactis HIS4 gene was cloned by complementation of a Saccharomyces cerevisiae his4 mutant. Sequence analysis revealed a 2388 bp open reading frame encoding a single polypeptide predicted to encompass three distinct enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase). This structural organization is strikingly similar to that of the His4 proteins from S. cerevisiae and Pichia pastoris. Transcript analysis detected a single mRNA species of 2.5 kb.