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Changing nucleosome positions in vivo through modification of the DNA rotational information
L Di Marcotullio1, M Buttinelli, G Costanzo
1Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, P. le Aldo Moro, 5 00185 Roma, Italy.
Abstract:
The effects of the rotational information of DNA in determining the in vivo localization of nucleosomal core particles (ncps) have been studied in the Saccharomyces cerevisiae 5 S rRNA repeat gene. The distribution of the phased series of flexibility signals present in this DNA has been altered by inserting in its centre a 25 bp tract. The effects of such alteration on the in vivo distribution of the helically phased, alternatively located ncps have been determined relative to a reference 21 bp insertion mutant. The results show that the answers provided in vitro and in vivo by the yeast 5 S rRNA gene sequence to specific modifications of the DNA rotational frame are similar, thus pointing to the relevance of DNA rotational information in vivo.