[Effects of progesterone on proliferation and differentiation of fetal rat calvarial osteoblasts in vitro]
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L Chen1, J Schøller, N T Foged
1Union Hospital, Tongji Medical University, Wuhan.
Objectives:
To investigate the influence of progesterone on proliferation and differentiation of osteoblast at the levels of gene expression and cell functions.
Methods:
Fetal rat calvarial osteoblasts were cultured in vitro in the presence of (10(-9) mol/L-10(-6) mol/L) progesterone. Cell proliferation, alkaline phosphalase (ALP) activity, osteocalcin mRNA expression and osteocalcin secretion in the medium and bone nodule formation were analyzed.
Results:
Progesterone did not influence cell proliferation; Progesterone enhanced the ALP activity in rat osteoblasts; Progesterone stimulated osteocalcin mRNA expression in a dose-dependent manner and increased the amount of osteocalcin in the culture medium; Progesterone increased both number and area of bone nodule formation.
Conclusion:
Progesterone has a multi-stimulating effect on the differentiation of fetal rat calvarial osteoblast, hut no effect on cell proliferation.
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