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[Effect of combining EGF with KGF on proliferation of human corneal epithelial cells]
1Zhongshan Ophthalmic Center, Sum Yat-sen University of Medical Sciences, Guangzhou, China.
Objective:
To select effective drugs for enhancing corneal epithelial wound healing and treating persistent corneal epithelial defect.
Methods:
Epidermal growth factor (EGF) and keratinocyte growth factor (KGF) were added to cultures of human corneal epithelial cells and DNA synthesis in corneal epithelial cells were measured with [3H]-thymidine incorporation and liquid scintillation techniques. The cells were counted in a hemocytometer to determine the doubling time.
Results:
10 ng/ml EGF, 10 ng/ml KGF enhanced cellular proliferation effectively (P < 0.01). EGF in combination with KGF, the enhancement was more effective (P < 0.05) and reduced the doubling time.
Conclusions:
Our results suggest that exogenous EGF with KGF can stimulate proliferation of human corneal epithelial cells and it may be of potential use clinically for treatment of persistent corneal epithelial
