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Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and
R M Wartell1, S Hosseini, S Powell
1School of Biology, Georgia Institute of Technology, Atlanta 30332, USA.
Journal of Chromatography. A
|June 26, 1998
Abstract:
Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting point mutations, and evaluating the effects of single site defects are also reported.