Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A second-step splicing activity is conserved from yeast to human

R M Cicarelli1, A Khaouja, C Codony

  • 1CID-CSIC, c/Jorge Girona Salgado 18-26, Barcelona, 08034, Spain.

Biochemical and Biophysical Research Communications
|June 27, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and implementation of novel liquid biopsy NGS panels via the OncNGS precommercial procurement (PCP) initiative.

ESMO open·2025
Same author

DNA-directed immobilization fluorescent immunoarray for multiplexed antibiotic residue determination in milk.

Analytical and bioanalytical chemistry·2024
Same author

Extended lower paratracheal lymph node resection during esophagectomy for cancer - safety and necessity.

BMC cancer·2022
Same author

Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2020
Same author

Ethylcellulose nanoparticles as a new "in vitro" transfection tool for antisense oligonucleotide delivery.

Carbohydrate polymers·2019
Same author

DNA-based nanoscaffolds as vehicles for 5-fluoro-2'-deoxyuridine oligomers in colorectal cancer therapy.

Nanoscale·2018

Yeast splicing factors can restore a defective human cell extract's second step of RNA splicing. This indicates that essential second-step splicing activity is conserved between yeast and human cells.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA splicing is a critical process for gene expression.
  • Defects in RNA splicing can lead to various diseases.
  • Understanding the conservation of splicing mechanisms is vital for deciphering cellular processes.

Purpose of the Study:

  • To investigate the conservation of the second step of RNA splicing between yeast and human cells.
  • To identify factors responsible for the second-step splicing activity.

Main Methods:

  • Utilized a defective HeLa nuclear extract deficient in the second step of splicing.
  • Tested the ability of yeast (Saccharomyces cerevisiae) fractions to restore the splicing activity.
  • Employed immunoaffinity chromatography using anti-trimethylguanosine antibodies to purify the active components.

Related Experiment Videos

Main Results:

  • A specific fraction from S. cerevisiae was found to restore the second-step splicing activity in the defective HeLa extract.
  • This yeast-derived activity demonstrates conservation of the second-step splicing mechanism.
  • The active component was identified as yeast U snRNP (small nuclear ribonucleoprotein) protein(s).

Conclusions:

  • The second step of RNA splicing is conserved between yeast and human cells.
  • Yeast U snRNP proteins possess conserved second-step splicing activity.
  • This finding provides insights into the evolutionary conservation of fundamental gene expression machinery.