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Mutants of Citrobacter freundii that transport and utilize melibiose
N Okazaki1, X J Xu, T Shimamoto
1Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Journal of Bacteriology
|June 27, 1998
Abstract:
We have isolated mutants of Citrobacter freundii that can grow on melibiose. Inducible alpha-galactosidase activity and melibiose transport activity were detected in the mutant cells but not in the wild-type cells. We detected a DNA region which hybridized with melB (the gene for the melibiose transporter) DNA of Escherichia coli in the chromosomal DNA of wild-type C. freundii. Protons, but not sodium ions, were found to be the coupling cations for melibiose (and methyl-beta-D-thiogalactoside) transport in the mutant cells.