Simultaneous determination of maltose and glucose using a screen-printed electrode system

Area of Science:

• Electrochemistry
• Biosensors
• Analytical Chemistry

Background:

• Simultaneous determination of sugars like maltose and glucose is crucial in various applications.
• Existing methods may lack specificity or require complex sample preparation.
• Development of rapid and accurate biosensing platforms is needed.

Purpose of the Study:

• To construct and optimize a screen-printed sensor system for simultaneous quantification of maltose and glucose.
• To evaluate the analytical performance and stability of the developed enzyme electrode system.

Main Methods:

• Fabrication of a screen-printed sensor with glucose oxidase (GOD) and amyloglucosidase/glucose oxidase (A/G) electrodes.
• Optimization of enzyme loading (20 units GOD/40 units amyloglucosidase), mediator concentration (0.2 mM 1,1'-ferrocenedimethanol), and hydroxyethyl cellulose (HEC) membrane composition.
• Determination of optimal pH (4.8) and linear ranges for glucose (up to 40 mM) and maltose (up to 20 mmol/L).

Main Results:

• The optimized sensor achieved simultaneous detection of maltose and glucose.
• Linear ranges were established up to 40 mM for glucose and 20 mmol/L for maltose.
• Coefficients of variation (CVs) ranged from 3.5% to 5.29%, indicating good precision.
• Results correlated well with the Fehling titration method.
• The enzyme electrodes exhibited good stability over four months when stored dry, particularly at 4°C.

Conclusions:

• A robust screen-printed sensor system for simultaneous maltose and glucose determination was successfully developed.
• The sensor offers high sensitivity, good precision, and excellent stability, making it suitable for practical applications.
• This biosensing approach provides an efficient alternative for sugar analysis in complex mixtures.