[Adhesiveness of peripheral blood neutrophils in normal subjects and in cancer patients]

A spectrophotometric method for assessing the capacity of phagocytes to adhere to the plastic is proposed. The method consists in staining a cell monolayer after Romanovsky-Giemsa in the wells of a 96-well flat-bottom plate, extraction of the stain, and assessment of the optic density of this solution at 650 nm. Methods for obtaining the cells in leukocyte concentrate (LC) and neutrophil suspension (NS) obtained in the density gradient are described. Two calibration curves for LC and NS are plotted for assessing the correspondence between optic density and a certain number of adhesive neutrophils. There were no statistical differences between the two curves. Neutrophil adhesion parameters of 38 donors are presented. The adhesive capacity of blood neutrophils is decreased in cancer patients (in 25 out of 27).