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Ceratobium mosaic potyvirus: another virus from orchids
A M Mackenzie1, M Nolan, K J Wei
1Research School of Biological Sciences, Australian National University, ACT, Australia.
Archives of Virology
|June 30, 1998
Summary
A new potyvirus, ceratobium mosaic virus, was found in Australian orchids. This virus is distinct from other known potyviruses, indicating a novel orchid pathogen requiring further study.
Area of Science:
- Plant virology
- Orchid diseases
- Molecular diagnostics
Background:
- Orchids are susceptible to various viral pathogens.
- Potyviruses are a significant genus of plant viruses with broad host ranges.
- Accurate detection and identification of plant viruses are crucial for disease management.
Purpose of the Study:
- To identify and characterize a novel potyvirus affecting orchids in Australia.
- To develop a molecular method for detecting the virus in orchid collections.
- To determine the phylogenetic relationship of the new virus to known potyviruses.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) using Potyviridae-specific primers.
- Direct nucleotide sequencing of RT-PCR products.
- Phylogenetic analysis of viral sequences.
Main Results:
- Ceratobium mosaic virus (CeMV) detected in approximately one-third of surveyed Australian orchids across 33 genera.
- RT-PCR with SP6/T7 promoter-tagged primers enabled direct sequencing of a ~1.7 kb viral genome fragment.
- Sequence analysis revealed CeMV represents a distinct potyvirus species, closely related to the bean common mosaic virus group but unique.
Conclusions:
- Ceratobium mosaic virus is a newly identified potyvirus infecting orchids in Australia.
- The developed RT-PCR method is effective for direct sequencing and identification of the virus.
- CeMV represents a novel species within the Potyviridae family, necessitating further research into its epidemiology and impact on orchids.