Related Experiment Videos
Ganglioside GM2 is substrate for a sialidase in MDCK cells
S Marchesini1, G Benaglia, A Piccinotti
1Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
Abstract:
GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5-24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence of fluorescent GM3 is consistent with the presence in these cells of a sialidase working on GM1 and GM2 gangliosides. After treatment of the cells with chloroquine the fluorescent GM1 remained essentially undegraded, indicating a catabolic processing at lysosomal level.