Related Experiment Video
Updated: Aug 9, 2026

Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells
Published on: August 1, 2013
Entry of African swine fever virus into Vero cells and uncoating
M L Valdeira1, C Bernardes, B Cruz
1Department of Cellular Biology, Faculty of Pharmacy, Lisboa, Portugal. lvaldeira@ff.ul.pt
Abstract:
African swine fever virus (ASFV) enters Vero cells by adsorptive endocytosis [Valdeira, M.L., Geraldes, A., 1985. Morphological study on the entry of African swine fever virus into cells, Biol Cell. 55, 35-40]. Electron microscopy of a lysosomotropic drug-controlled penetration indicated that this step takes place in the endosomes, after fusion between the viral envelope and the limiting membrane of the endosome. Inhibition studies with colcemid, cytochalasin B, sodium azide, dinitrophenol, lysosomotropic weak bases, and the ionophore monensin, showed that the virus uptake is largely independent of cytoskeletal and lysosomal function, but dependent on oxidative phosphorylation. Some protease inhibitors inhibited viral replication at an early step, indicating that the initiation of infection depends on a viral proteolytic cleavage.
Related Concept Videos
What are Viruses?
Retrovirus Life Cycles
Viral Recombination
Inhibitors Of Virion Release
Inhibitors of Virion Maturation and Assembly

