Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA editing: trypanosomes rewrite the genetic code

K Stuart

    Verhandelingen - Koninklijke Academie Voor Geneeskunde Van Belgie
    |July 1, 1998
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Validation of the Measure of Case-Discussion Complexity and the Metric for the Observation of Decision-Making for Streamlining Workflow and Evaluating Decision-Making in US Tumor Boards.

    Cancer medicine·2026
    Same author

    Definitive Stereotactic Body Radiation Therapy in Early-Stage Solitary Hepatocellular Carcinoma: An Australian Multi-Institutional Review of Outcomes.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2023
    Same author

    Efficacy and Toxicity of Stereotactic Body Radiotherapy for Early to Advanced Stage Hepatocellular Carcinoma - Initial Experience From an Australian Liver Cancer Service.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2020
    Same author

    Predicting inpatient hypoglycaemia in hospitalized patients with diabetes: a retrospective analysis of 9584 admissions with diabetes.

    Diabetic medicine : a journal of the British Diabetic Association·2017
    Same author

    You can't get there from here: misplaced incentives can undermine the goals of health care reform in the NICU setting.

    Journal of perinatology : official journal of the California Perinatal Association·2012
    Same author

    A case of chronic ruminal tympany.

    The Veterinary record·2010
    Same journal

    Air pollution in Belgium: will we be able to comply with the European standards?

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie·2012
    Same journal

    The influence of air pollution on health: mechanistic implications.

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie·2012
    Same journal

    Air pollution and health--air quality standards.

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie·2012
    Same journal

    [Worry about talent in first line health care].

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie·2012
    Same journal

    [Piet de Somer, the University of Leuven and the Belgium poliovaccine in 1956-57].

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie·2012
    Same journal

    [Emergency guidelines for cosmetic procedures and esthetic skin treatment: recommendations of the medical establishment].

    Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie·2012
    See all related articles

    Genetic information in trypanosomes is complex, with messenger RNA (mRNA) sequences extensively modified by RNA editing guided by guide RNAs (gRNAs). This process remodels RNA, enabling precise protein synthesis despite dispersed gene information.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Parasitology

    Background:

    • The central dogma of molecular biology describes DNA to RNA to protein information flow.
    • Exceptions include RNA viruses, reverse transcriptase, introns, alternative splicing, and RNA editing.
    • Trypanosome parasites exhibit unique genetic complexity involving RNA editing.

    Purpose of the Study:

    • To investigate the mechanism and regulation of RNA editing in trypanosomes.
    • To understand how guide RNAs (gRNAs) direct the precise sequence of edited mRNA.
    • To characterize the enzymatic machinery involved in RNA editing.

    Main Methods:

    • Development of an in vitro system to study RNA editing.
    • Characterization of partially edited RNAs to determine editing directionality.

    Related Experiment Videos

  • Purification of the multi-enzyme complex responsible for editing.

    • Main Results:

    • RNA editing in trypanosomes extensively remodels mRNA sequences, often obscuring original gene identity.
    • Editing is guided by numerous gRNAs, each encoded by a separate gene.
    • Editing involves a series of enzymatic steps and is performed by a multi-protein complex.
    • Differential editing regulates energy metabolism during the parasite's life cycle.

    Conclusions:

    • RNA editing in trypanosomes is a crucial process for generating functional mRNAs from dispersed genetic information.
    • The mechanism involves precise gRNA-guided uridylate insertion/deletion.
    • The multi-enzyme editing complex is a target for further mechanistic studies.
    • RNA editing plays a vital role in regulating parasite biology and energy metabolism.