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Related Experiment Videos

Cytochrome c oxidase: structure and spectroscopy

H Michel1, J Behr, A Harrenga

  • 1Max-Planck-Institut für Biophysik, Frankfurt/Main, Germany. michel@mbibp-frankfurt.mpg.de

Annual Review of Biophysics and Biomolecular Structure
|July 1, 1998
PubMed
Summary
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Cytochrome c oxidase, crucial for cellular energy, transfers electrons to oxygen, creating a proton gradient for ATP synthesis. Recent structural and spectroscopic studies reveal key mechanisms of this vital enzyme.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Cytochrome c oxidase is the terminal enzyme in respiratory chains of mitochondria and aerobic bacteria.
  • It catalyzes the reduction of oxygen to water, coupled with proton translocation across membranes.
  • This process generates a proton gradient essential for ATP synthesis by F0F1-ATPase.

Purpose of the Study:

  • To review recent advancements in understanding cytochrome c oxidase structure and function.
  • To integrate structural and spectroscopic data to elucidate enzyme mechanisms.
  • To focus on oxygen intermediates, proton transfer pathways, and the coupling of electron transfer to proton translocation.

Main Methods:

  • Spectroscopic techniques including EPR, absorbance, and resonance Raman spectroscopy.

Related Experiment Videos

  • Site-directed mutagenesis to identify key residues.
  • Analysis of recently determined crystal structures of bacterial and mitochondrial cytochrome c oxidase.

    • Main Results:

    • Elucidation of cofactor and ligand nature.
    • Identification of residues critical for proton transfer.
    • Insights into the enzyme's catalytic cycle and intermediate structures.
    • Determination of crystal structures providing high-resolution views of the enzyme.

    Conclusions:

    • Recent structural and spectroscopic studies have significantly advanced the understanding of cytochrome c oxidase.
    • Mechanistic aspects, including oxygen intermediates and proton transfer pathways, are better understood.
    • The coupling mechanism between electron transfer and proton translocation remains an active area of research.