Determination of ascorbic acid in nanolitre samples by means of capillary batch injection analysis
Related Experiment Video
Updated: Oct 1, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
U Backofen1, F M Matysik, W Hoffmann
1Forschungszentrum Karlsruhe, Institut für Instrumentelle Analytik, Germany.
Abstract:
Capillary batch injection analysis (CBIA) is applied to the determination of ascorbic acid in nanolitre samples at neutral pH. It is demonstrated that pulsed amperometric detection is superior to constant-potential amperometric detection with respect to signal stability. The calibration plot is linear within the studied concentration range between 0.5 mM and 5 mM and the reproducibility is characterized by a relative standard deviation of 0.80% for 10 successive injections of 188 nL of 3.5 mM ascorbic acid. The attainable sample throughput is up to 500 per hour.
More Related Videos
10:22Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
Published on: June 30, 2020
08:54Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector
Published on: May 30, 2025