Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative sperm mucus penetration: modified formulae for calculating penetration efficiency

G N Clarke1, C Garrett, H W Baker

  • 1Andrology Unit, The Royal Women's Hospital, Carlton, Australia.

Human Reproduction (Oxford, England)
|July 1, 1998
PubMed
Summary

This study refined the sperm-cervical mucus penetration efficiency measure by correcting for sperm depletion. Model II, assuming a select subpopulation of sperm penetrates, proved more accurate and independent of mucus column size.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measuring bonding or attachment in the parent-infant-relationship: A systematic review of parent-report assessment measures, their psychometric properties and clinical utility.

Clinical psychology review·2020
Same author

STRAWB2 (Stress and Wellbeing After Childbirth): a randomised controlled trial of targeted self-help materials to prevent post-traumatic stress disorder following childbirth.

BJOG : an international journal of obstetrics and gynaecology·2020
Same author

Belatacept Combined With Transient Calcineurin Inhibitor Therapy Prevents Rejection and Promotes Improved Long-Term Renal Allograft Function.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
Same author

Prevention of Depression in At-Risk Adolescents: Moderators of Long-term Response.

Prevention science : the official journal of the Society for Prevention Research·2016
Same author

Retreatment with anti-EGFR based therapies in metastatic colorectal cancer: impact of intervening time interval and prior anti-EGFR response.

BMC cancer·2015
Same author

Characterizing the patterns of clonal selection in circulating tumor DNA from patients with colorectal cancer refractory to anti-EGFR treatment.

Annals of oncology : official journal of the European Society for Medical Oncology·2015

Area of Science:

  • Reproductive Biology
  • Sperm Motility Analysis
  • Biophysics

Background:

  • The Percentage of Successful Collisions (PSC) formula by Katz et al. (1980) quantified sperm-cervical mucus penetration efficiency.
  • PSC's validity declined due to reported values >100% and variability with mucus column cross-sectional area.

Purpose of the Study:

  • To develop a more accurate measure of sperm-cervical mucus penetration efficiency.
  • To correct the original PSC formula for sperm depletion effects in the semen reservoir.

Main Methods:

  • Derived two modified PSC formulae based on different sperm-mucus interaction models.
  • Model (i): equal penetration chance per motile spermatozoon.
  • Model (ii): select subpopulation penetrates with 100% efficiency.

Related Experiment Videos

Main Results:

  • Both modified formulae yielded higher PSC values than original estimates.
  • Depletion corrections ranged from 4-46% (model i) and 190-320% (model ii).
  • Model (ii) results were invariant to capillary cross-sectional area, unlike model (i).

Conclusions:

  • Model (ii) assumptions provide a more accurate description of sperm-mucus interaction.
  • The corrected PSC accounts for sperm depletion, improving accuracy in penetration efficiency measurement.
  • This refined method offers better insights into sperm function and reproductive potential.