Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spontaneous conversion of L-dehydroascorbic acid to L-ascorbic acid and L-erythroascorbic acid

C H Jung1, W W Wells

  • 1Department of Biochemistry, Michigan State University, East Lansing, Michigan, 48824, USA.

Archives of Biochemistry and Biophysics
|July 2, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy and safety of combination therapy with SGLT2 and DPP4 inhibitors in the treatment of type 2 diabetes: A systematic review and meta-analysis.

Diabetes & metabolism·2018
Same author

Family history of diabetes and the risk of subclinical atherosclerosis.

Diabetes & metabolism·2015
Same author

Serum bilirubin as a predictor of incident metabolic syndrome: a 4-year retrospective longitudinal study of 6205 initially healthy Korean men.

Diabetes & metabolism·2014
Same author

The optimal morning:evening ratio in total dose of twice-daily biphasic insulin analogue in poorly controlled Type 2 diabetes: a 24-week multi-centre prospective, randomized controlled, open-labelled clinical study.

Diabetic medicine : a journal of the British Diabetic Association·2013
Same author

Successful endoscopic ultrasound-guided ethanol ablation of multiple insulinomas accompanied with multiple endocrine neoplasia type 1.

Internal medicine journal·2013
Same author

Clinical characteristics and insulin independence of Koreans with new-onset type 2 diabetes presenting with diabetic ketoacidosis.

Diabetes/metabolism research and reviews·2013

Dehydroascorbic acid spontaneously decomposes into L-erythroascorbic acid, a vitamin C analog. This finding is crucial for understanding dehydroascorbic acid metabolism and its potential interference in related studies.

Area of Science:

  • Biochemistry
  • Nutritional Science
  • Analytical Chemistry

Background:

  • Dehydroascorbic acid (DHA) is the oxidized form of ascorbic acid (vitamin C).
  • DHA decomposition at neutral and alkaline pH can yield various products.
  • Understanding DHA metabolism is vital for nutritional and biochemical research.

Purpose of the Study:

  • To identify and characterize the decomposition products of dehydroascorbic acid.
  • To investigate factors influencing the formation of these products.
  • To develop methods for studying dehydroascorbic acid metabolism.

Main Methods:

  • High-performance liquid chromatography (HPLC) with electrochemical detection.
  • Reversed-phase HPLC for compound isolation.

Related Experiment Videos

  • Mass spectrometry and UV spectroscopy for compound identification.

    • Main Results:

    • Dehydroascorbic acid spontaneously decomposed into three products at neutral/alkaline pH.
    • Ascorbic acid was identified as one decomposition product.
    • A major new compound, identified as L-erythroascorbic acid, was formed.
    • Fe(II), Cu(I), phosphate, and cyanide influenced L-erythroascorbic acid production.
    • Desferrioxamine inhibited L-erythroascorbic acid formation.

    Conclusions:

    • Dehydroascorbic acid can be reduced to ascorbic acid without exogenous reductants.
    • L-erythroascorbic acid is a significant decomposition product of dehydroascorbic acid.
    • The identification and quantitation of L-erythroascorbic acid offer a new tool for studying dehydroascorbic acid metabolism.