Related Experiment Videos
Structural characterization and mapping of the normal epithelial cell-specific 1 gene
L Luo1, J A Herbrick, S W Scherer
1Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada.
Biochemical and Biophysical Research Communications
|July 2, 1998
Summary
The normal epithelial cell-specific 1 (NES1) gene, implicated in breast cancer, has been structurally characterized. Its genomic location on chromosome 19q13.3-q13.4 places it near the kallikrein gene family.
Area of Science:
- Genomics
- Molecular Biology
- Cancer Research
Background:
- The normal epithelial cell-specific 1 (NES1) gene is a novel serine protease-like gene.
- NES1 is down-regulated during breast cancer progression.
- Its genomic structure and chromosomal location were previously unknown.
Purpose of the Study:
- To characterize the genomic structure of the NES1 gene.
- To determine the chromosomal position of the NES1 gene.
Main Methods:
- Subcloning and sequencing of a PAC clone containing the NES1 gene.
- Analysis of intron-exon structure.
- Somatic cell mapping, radiation hybrid mapping, and fluorescence in situ hybridization for gene localization.
Main Results:
- The NES1 gene spans 5.5 kb and consists of five coding exons and one untranslated exon.
- Intron positions are similar to trypsinogen, prostate specific antigen (PSA), and tissue plasminogen activator (TPA).
- NES1 was mapped to chromosome 19q13.3-q13.4, a region housing the human kallikrein gene family.
Conclusions:
- The NES1 gene shares structural similarities and evolutionary origins with trypsinogen, PSA, and TPA.
- NES1 is located in close proximity to the PSA gene on chromosome 19.
- These findings provide insights into the genomic organization and evolutionary relationships of NES1.