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Related Experiment Videos

Cell line availability: where to get the cell lines you need

R J Hay1

  • 1American Type Culture Collection, Cell Culture Department, Rockville, Maryland 20852, USA.

Methods in Cell Biology
|July 2, 1998
PubMed
Summary

This study reviews cell line availability and quality control, highlighting the need for rigorous testing to prevent microbial contamination and cross-contamination in cell cultures.

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Area of Science:

  • Cell Biology
  • Microbiology

Background:

  • Cell lines are crucial tools in biological research.
  • Maintaining the integrity and purity of cell lines is essential for reproducible results.

Purpose of the Study:

  • To summarize the availability and utility of cell lines with varying doubling potentials.
  • To emphasize the critical need for stringent quality control in cell culture.

Main Methods:

  • Literature review of cell line availability and utility.
  • Compilation of contact information for national cell banks.
  • Analysis of data on microbial infection and cross-contamination frequencies.

Main Results:

  • Cell lines with limited and continuous doubling potential are available.
  • National cell banks provide essential resources.
  • High incidences of microbial contamination and cross-contamination are prevalent in cultured cell lines.

Conclusions:

  • Vigorous quality control procedures are imperative for reliable cell line usage.
  • Preventing microbial contamination and cross-contamination is vital for research integrity.

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