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Controlled insulin release from chitosan microparticles

F Bugamelli1, M A Raggi, I Orienti

  • 1Department of Pharmaceutical Sciences, University of Bologna, Italy.

Archiv Der Pharmazie
|July 2, 1998
PubMed
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Chitosan microparticles loaded with insulin were successfully produced using ascorbyl palmitate as a crosslinker, ensuring efficient drug release. Alternative crosslinkers resulted in incomplete drug release and variable release rates.

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Chitosan is a versatile biopolymer with potential in drug delivery.
  • Developing efficient methods for encapsulating therapeutic proteins like insulin is crucial.
  • Interfacial crosslinking offers a promising approach for microparticle fabrication.

Purpose of the Study:

  • To develop and characterize insulin-loaded chitosan microparticles using interfacial crosslinking.
  • To investigate the role of ascorbyl palmitate as an effective interfacial crosslinker.
  • To compare the drug release profiles of microparticles prepared with different crosslinking agents.

Main Methods:

  • Preparation of chitosan microparticles via interfacial crosslinking in a water/oil dispersion.

Related Experiment Videos

  • Utilizing ascorbyl palmitate as an amphiphilic crosslinker at the water/oil interface.
  • Characterization of microparticle drug loading and release kinetics using spectrophotometric and spectrofluorimetric methods.

    • Main Results:

    • Microparticles prepared with ascorbyl palmitate exhibited high insulin loading and complete drug release over approximately 80 hours at a constant rate.
    • Replacing ascorbyl palmitate with dehydroascorbyl palmitate led to incomplete drug release and non-constant release rates.
    • The amphiphilic nature of ascorbyl palmitate is critical for efficient interfacial crosslinking and controlled drug release.

    Conclusions:

    • Ascorbyl palmitate is an effective interfacial crosslinker for producing insulin-loaded chitosan microparticles with desirable release characteristics.
    • The choice of crosslinker significantly impacts microparticle properties, drug loading, and release kinetics.
    • Spectrophotometric and spectrofluorimetric methods are suitable for evaluating insulin stability and release from chitosan microparticles.