Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative analysis of amelogenin solubility

J Tan1, W Leung, J Moradian-Oldak

  • 1Center for Craniofacial Molecular Biology, University of Southern California, School of Dentistry, Los Angeles 90033, USA.

Journal of Dental Research
|July 2, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triple Function of Amelogenin Peptide-Chitosan Hydrogel for Dentin Repair.

Journal of dental research·2023
Same author

Biomineralization of Enamel and Dentin Mediated by Matrix Proteins.

Journal of dental research·2021
Same author

An Evolutionarily Conserved Helix Mediates Ameloblastin-Cell Interaction.

Journal of dental research·2020
Same author

The Presence of MMP-20 Reinforces Biomimetic Enamel Regrowth.

Journal of dental research·2017
Same author

Amelogenin-Ameloblastin Spatial Interaction around Maturing Enamel Rods.

Journal of dental research·2016
Same author

Extracts of irradiated mature human tooth crowns contain MMP-20 protein and activity.

Journal of dentistry·2014

Amelogenin solubility varies with pH, ionic strength, and protein structure. Low solubility under physiological conditions may stem from amelogenins forming aggregates.

Area of Science:

  • Biochemistry
  • Biomaterials Science
  • Dental Enamel Research

Background:

  • Amelogenins are key extracellular enamel matrix proteins.
  • They are crucial for regulating enamel crystal formation and size.
  • Understanding amelogenin solubility is vital for enamel development research.

Purpose of the Study:

  • To compare the solubility of various amelogenins across a pH range (4.0-9.0).
  • To investigate the impact of buffer composition, ionic strength, and divalent metal ions on solubility.
  • To elucidate factors influencing amelogenin behavior in solution.

Main Methods:

  • Solubility assays were performed on recombinant and native amelogenins.
  • Experiments were conducted at varying pH levels and constant ionic strength.

Related Experiment Videos

  • The influence of buffer composition, ionic strength, and metal ions (Ca2+, Mg2+, Zn2+) was examined.

    • Main Results:

    • Amelogenin solubility was minimal near its isoelectric point, increasing at higher and lower pH.
    • Different amelogenin variants (e.g., rM179, 25K, 23K, rM166, TRAP, LRAP) exhibited distinct solubility profiles.
    • Increased ionic strength decreased solubility, while Zn2+ reduced it, but Ca2+ and Mg2+ had no significant effect.

    Conclusions:

    • Amelogenin solubility is significantly influenced by primary structure, solution pH, and ionic strength.
    • The observed low solubility under physiological conditions suggests a propensity for in vivo aggregation.
    • These findings contribute to understanding enamel matrix formation and potential biomaterial applications.