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The human autoantigen La/SS-B accelerates herpes simplex virus type 1 replication in transfected mouse 3T3 cells
M Bachmann1, H Deister, A Pautz
1Institut für Physiologische Chemie, Johannes-Gutenberg Universität, Mainz, Germany.
Clinical and Experimental Immunology
|July 2, 1998
Summary
The human La/SS-B protein translocates from the nucleus to the cytoplasm during herpes simplex virus type 1 infection. Higher La/SS-B expression accelerates viral replication, indicating its role as a cellular factor.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- The human autoantigen La/SS-B is involved in various cellular processes.
- Herpes simplex virus type 1 (HSV-1) infection impacts host cell machinery.
Purpose of the Study:
- To investigate the role of human La/SS-B protein during HSV-1 infection.
- To determine if La/SS-B influences viral replication dynamics.
Main Methods:
- Transfected mouse cell lines expressing varying levels of human La/SS-B were infected with HSV-1 strains.
- Immunofluorescence using a human-specific anti-La monoclonal antibody tracked La protein localization.
- Viral replication rates were quantified in relation to La/SS-B expression levels.
Main Results:
- Human La protein translocated from the nucleus to the cytoplasm post-HSV-1 infection.
- The kinetics of La protein translocation were dependent on its expression level.
- Increased La/SS-B expression correlated with accelerated HSV-1 replication.
Conclusions:
- Human La/SS-B protein facilitates HSV-1 replication.
- La/SS-B acts as a cellular factor that enhances virus propagation.