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Anion competition for a volume-regulated current

I Levitan1, S S Garber

  • 1Department of Physiology, Allegheny University of Health Sciences, Philadelphia, Pennsylvania 19129, USA.

Biophysical Journal
|July 2, 1998
PubMed
Summary
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Anionic amino acids, glutamate and aspartate, share volume-regulated channels with chloride ions. Their permeation is interdependent, suggesting channels can hold multiple ions, impacting cell volume regulation.

Area of Science:

  • Cellular physiology
  • Ion channel biophysics
  • Molecular neuroscience

Background:

  • Cell volume regulation is crucial for cellular homeostasis.
  • Volume-regulated anion channels (VRACs) play a key role in this process.
  • The specific permeants and mechanisms of VRACs are not fully understood.

Purpose of the Study:

  • To investigate if anionic amino acids (glutamate, aspartate) share VRACs with chloride ions.
  • To determine the interdependence of ion permeation through VRACs.
  • To elucidate the role of ion interactions in volume regulation.

Main Methods:

  • Whole-cell patch-clamp recordings in hyposmotic conditions to induce cell swelling.
  • Measurement of volume-regulated anion currents carried by chloride, glutamate, and aspartate.

Related Experiment Videos

  • Analysis of permeability ratios and current amplitudes across varying mole fraction ratios of permeant anions.

    • Main Results:

    • Anionic amino acids and chloride ions permeate through the same volume-regulated conductance.
    • Permeation of amino acids and chloride ions are interdependent, indicating simultaneous channel occupancy.
    • Eyring rate theory suggests distinct permeation barriers for chloride (intracellular) and amino acids (extracellular).

    Conclusions:

    • VRACs can accommodate multiple ions, including anionic amino acids and chloride, concurrently.
    • Interactions between permeant ions within VRACs may modulate cell volume regulation.
    • Findings provide insights into the molecular mechanisms and physiological relevance of VRAC function.