Related Experiment Video
Updated: Jun 25, 2026
10:24
Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
Published on: December 17, 2012
Three distinct messenger RNA distribution patterns in human jejunal enterocytes
W Li1, S D Krasinski, M Verhave
1Department of Pediatrics, Floating Hospital for Children, Boston, Massachusetts, USA.
Gastroenterology
|July 3, 1998
Summary
Messenger RNA (mRNA) localization in human enterocytes is crucial. This study reveals distinct mRNA sorting patterns for villin, sodium glucose cotransporter 1, and liver fatty acid-binding protein, indicating active mechanisms.
Area of Science:
- Cell Biology
- Molecular Biology
- Gastroenterology
Background:
- Messenger RNA (mRNA) localization in human enterocytes is not well understood.
- Previous research suggests asymmetric mRNA distribution in intestinal epithelial cells.
- The relationship between mRNA and encoded protein localization is generally assumed to be concordant.
Purpose of the Study:
- To characterize mRNA localization patterns in human enterocytes for three genes with diverse functions.
- To investigate the mechanisms of mRNA sorting in intestinal epithelial cells.
- To determine if mRNA distribution correlates with protein distribution.
Main Methods:
- In situ hybridization using digoxigenin-labeled RNA probes.
- Analysis of mRNA distribution in human jejunum tissue sections.
- Microscopic examination of enterocytes to determine mRNA localization.
Main Results:
- Villin mRNA was localized to the basal region of enterocytes.
- Sodium glucose cotransporter 1 mRNA was found in the apical region.
- Human liver fatty acid-binding protein mRNA showed diffuse cytoplasmic distribution.
Conclusions:
- Distinct mRNA distribution patterns suggest active mRNA sorting mechanisms in human enterocytes.
- This study provides evidence for potential dichotomies between mRNA and protein localization.
- Understanding mRNA localization is key to deciphering cellular function in the intestine.
More Related Videos
11:19Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Published on: September 30, 2016
12:15The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
Published on: October 3, 2017
Related Concept Videos
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
02:22Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
01:29Insertion of Multi-pass Transmembrane Proteins in the RER
The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
02:22Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...