Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular interaction between factor VII and tissue factor

S Higashi1, S Iwanaga

  • 1Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Japan. shigashi@cserv2.yokohama-cu.ac.jp

International Journal of Hematology
|July 3, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Relationship Among Establishment Durations, Kin Relatedness, Aggressiveness, and Distance Between Populations of Eight Invasive Argentine Ant (Hymenoptera: Formicidae) Supercolonies in Japan.

Journal of economic entomology·2017
Same author

Effects of inflorescence size on visits from pollinators and seed set of Corydalis ambigua (Papaveraceae).

Oecologia·2017
Same author

Defense of termitaria by termitophilous ants.

Oecologia·2017
Same author

Serum levels of IL-6 and IL-1β can predict the efficacy of gemcitabine in patients with advanced pancreatic cancer.

British journal of cancer·2013
Same author

Ultraselective transcatheter infusion of epirubicin in water-in-oil-in-water emulsion for small hepatocellular carcinoma.

The British journal of radiology·2012
Same author

[Graft replacement for surgical repair of coarctation of the aorta in adults].

Kyobu geka. The Japanese journal of thoracic surgery·2011
Same journal

Long-term treatment-free remission in CML after early dasatinib discontinuation due to interstitial pneumonitis.

International journal of hematology·2026
Same journal

Correction to: Low reticulocyte count at infusion is a risk factor for high-grade cytokine release syndrome in chimeric antigen receptor T cell therapy.

International journal of hematology·2026
Same journal

The efficacy of prophylactic tranexamic acid in patients with hematologic malignancy: a systematic review and meta-analysis.

International journal of hematology·2026
Same journal

Current status and challenges of TCR-T cell therapy for AML/MDS.

International journal of hematology·2026
Same journal

Efficacy and safety of maribavir in Japanese patients with post-transplant cytomegalovirus infections: a phase III study.

International journal of hematology·2026
Same journal

Prognostic impact of WT1 dynamics in peripheral blood before and after allogeneic HSCT in patients with AML and MDS.

International journal of hematology·2026
See all related articles

The interaction between factor VIIa (VIIa) and tissue factor (TF) significantly boosts blood coagulation. TF binding to VIIa induces conformational changes, enhancing its catalytic activity and initiating the extrinsic coagulation pathway.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Background:

  • Factor VIIa (VIIa) and tissue factor (TF) form a complex crucial for extrinsic blood coagulation initiation.
  • The crystal structure of the VIIa-TF complex reveals detailed interacting surfaces.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying TF-mediated enhancement of VIIa catalytic activity.
  • To identify key regions of VIIa and TF involved in their high-affinity interaction.

Main Methods:

  • X-ray crystallography to determine complex structure.
  • Mutational and biochemical studies to identify essential interaction regions.
  • Chemical modification studies to model TF-accelerated VIIa activity.

Main Results:

Related Experiment Videos

  • VIIa interacts with distinct TF sites via its gamma-carboxyglutamic acid, EGF-like, and protease domains.
  • TF binding to VIIa's protease domain induces a conformational shift in the active site.
  • A model proposes TF shifts VIIa from an inactive to an active state, accelerating its activity.

Conclusions:

  • The VIIa-TF interaction involves multiple mechanisms enhancing coagulant activity, vital for initiating blood coagulation.
  • Understanding VIIa-TF interactions aids in comprehending coagulation regulation and designing anticoagulants for thrombotic diseases.