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Updated: Dec 20, 2025
Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
Published on: June 7, 2019
Ultracytochemical analysis of E-PTA-positive synaptic junctions in postmortem-examined brains with neurologic
A Tateno1, K Inomata, T Makifuchi
1Department of Pediatrics, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.
Abstract:
The synaptic junctions from four postmortem-examined brains were studied ultracytochemically, using the ethanolic phosphotungustic acid (E-PTA) method. A noteworthy finding was the presence of variable-shaped vesicles that were not observed in the control E-PTA-treated preparations. This structural change in synaptic junctions is thought to represent a degenerative process. It is suggested that the neuronal transmission in brains with acquired neuropathologic abnormalities may be impaired because of the degenerative change in synaptic junctions.
