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Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA
T Yeghiazarian1, Y Zhao, S E Read
1Chiron Diagnostics, Emeryville, California 94608-2916, USA. tory_yeghiazarian@cc.chiron.com
Journal of Clinical Microbiology
|July 3, 1998
Abstract:
We have developed small-volume (50 or 250 microl)-format branched-DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.
