Role of calcium oscillations in mammalian egg activation: experimental approach

Biological rhythms are everywhere; the pulsatility of intracellular signals appears to maximise the cellular processes better than constant signaling. The aim of this paper is, firstly, to review the cellular mechanisms that modulate calcium oscillator activity during fertilisation and, secondly, to describe recent results we have obtained by artificially imposing rhythmical calcium stimulation on fertilised rabbit eggs during in vitro culture. The key finding in these experiments is that the egg appears to be sensitive to repetitive signalling during a period that goes far beyond the time of meiosis reinitiation. When delivered at the proper rhythm transient signalling can optimise developmental processes.