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Related Experiment Videos

Control of interferon-tau gene expression by Ets-2

T Ezashi1, A D Ealy, M C Ostrowski

  • 1Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA.

Proceedings of the National Academy of Sciences of the United States of America
|July 8, 1998
PubMed
Summary

Transcription factor Ets-2 is crucial for interferon-tau (IFN-tau) gene expression in early pregnancy trophectoderm. This study identified Ets-2 and GABPalpha as key regulators, highlighting Ets-2

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Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Developmental biology

Background:

  • Interferon-tau (IFN-tau) genes are transiently expressed in ruminant embryonic trophectoderm during early pregnancy.
  • Conserved promoter regions, particularly a proximal sequence (bp -91 to -69), are critical for trophoblast-specific expression.

Purpose of the Study:

  • To identify transcription factors regulating trophoblast-specific IFN-tau gene expression.
  • To elucidate the role of the Ets family in controlling IFN-tau gene activity.

Main Methods:

  • Yeast one-hybrid screening of a day 13 conceptus cDNA library using the IFN-tau proximal promoter sequence.
  • Reporter gene assays (luciferase) involving cotransfection of promoter constructs and transcription factor expression plasmids in human JAr cells.

Related Experiment Videos

  • Immunocytochemical detection of Ets-2 in embryonic trophectoderm nuclei.

    • Main Results:

    • Ets-2 and GABPalpha were identified as transcription factors binding to the IFN-tau proximal promoter.
    • Active ovine IFN-tau genes possess a GGAA Ets motif, while inactive genes have a mutated TGAA motif.
    • Ets-2 significantly transactivated the promoter of an active bovine IFN-tau gene (boIFNT1) in JAr cells, while inactive gene promoters were unaffected.
    • GABPalpha showed minimal effect alone but a modest increase with GABPbeta.
    • Ets-2 was confirmed in embryonic trophectoderm nuclei.

    Conclusions:

    • Ets-2 is a key regulator involved in the transient, nonvirally inducible expression of IFN-tau genes.
    • The identified Ets motif in the promoter is critical for Ets-2 mediated transactivation.
    • These findings provide insights into the molecular mechanisms governing early embryonic gene expression in ruminants.