Bis-diazotized benzidine as a spray reagent for detecting zearalenone on thin layer chromatoplates

Bis-diazotized benzidine is a highly sensitive spray reagent for detecting zearalenone (F-2) in corn samples. As little as 2.0 ng of this Fusarium toxin could be visualized on a thin layer plate after development. In addition, this spray reagent is more specific than the spray reagents currently used for zearalenone.