Related Experiment Video
Updated: Aug 15, 2026

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Published on: October 6, 2022
Determination of pravastatin in human plasma by high-performance liquid chromatography with ultraviolet detection
Abstract:
A sensitive high-performance liquid chromatographic (HPLC) method for the quantitation of the HMG-CoA reductase inhibitor pravastatin in human plasma is described. Sample preparation based on reversed-phase solid-phase extraction using triamcinolone acetonide as internal standard (I.S.). The compounds were separated on C18 reversed-phase analytical column and then determined by ultraviolet detection. The recovery of pravastatin from plasma was 69.2+/-6.7% (mean+/-S.D.). The limit of detection for pravastatin in aqueous solution was 0.4 ng, the limit of quantitation in plasma was 2 ng/ml. In a preliminary pharmacokinetic study with two healthy volunteers the t1/2 of pravastatin in plasma was found to be 0.8 and 2.3 h.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Types of Detectors
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...

