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Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

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Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
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Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
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Related Experiment Video

Updated: May 5, 2026

Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
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Urate crystal-induced chemotactic factor: isolation and partial characterization

I Spilbert, A Gallacher, J M Mehta

    The Journal of Clinical Investigation
    |October 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified a heat-labile glycoprotein (8,400 daltons) from phagocytosed urate crystals that attracts neutrophils and mononuclear cells. This factor also triggers lysosomal enzyme release in human neutrophils.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Neutrophils and mononuclear cells are key immune cells involved in inflammatory responses.
    • Phagocytosis of monosodium urate crystals can trigger inflammatory processes, particularly in conditions like gout.

    Purpose of the Study:

    • To identify and characterize a chemotactic factor released by neutrophils after phagocytosing monosodium urate crystals.
    • To investigate the properties and biological activity of this identified chemotactic factor.

    Main Methods:

    • Extraction of a chemotactic factor from the lysosomal fraction of human and rabbit neutrophils that had phagocytosed monosodium urate crystals.
    • Characterization of the factor's molecular weight, heat lability, and species-specific activity.
    • Assessment of the factor's effect on cell migration and lysosomal enzyme release.

    Main Results:

    • A heat-labile glycoprotein with a molecular weight of 8,400 daltons was isolated, exhibiting chemotactic properties for both human and rabbit neutrophils and mononuclear cells.
    • Preincubation with the urate-induced chemotactic factor or complement-activated plasma inhibited subsequent chemotactic migration.
    • The chemotactic factor stimulated the release of lysosomal enzymes in cytochalasin B-treated human neutrophils.

    Conclusions:

    • A novel chemotactic factor derived from urate crystal phagocytosis by neutrophils plays a role in immune cell recruitment.
    • This factor's ability to induce lysosomal enzyme release suggests its involvement in inflammatory mediator secretion.
    • Understanding this factor may offer insights into the pathogenesis of crystal-induced inflammation and potential therapeutic targets.