Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An in vitro model for acoustic overstimulation

A Fridberger1, J T van Maarseveen, M Ulfendahl

  • 1Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden. anders.fridberger@fyfa.ki.se

Acta Oto-Laryngologica
|July 9, 1998
PubMed
Summary

This study developed an in vitro model to investigate acoustic trauma effects on cochlear hair cells. While high-intensity sound stimulation caused cell vibrations and stiffness changes, most cells showed remarkable resistance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outer hair cell driven reticular lamina mechanical distortion in living cochleae.

Hearing research·2021
Same author

Lateral Diffusion Anisotropy and Membrane Lipid/Skeleton Interaction in Outer Hair Cells.

Biophysical journal·2018
Same author

Increased vitamin plasma levels in Swedish military personnel treated with nutrients prior to automatic weapon training.

Noise & health·2011
Same author

Confocal slit divided-aperture microscope: applications in ear research.

Applied optics·2010
Same author

Differential gene expression in the rat cochlea after exposure to impulse noise.

Neuroscience·2006
Same author

Lateral diffusion anisotropy and membrane lipid/skeleton interaction in outer hair cells.

Biophysical journal·2006

Area of Science:

  • Otoacoustic emissions
  • Auditory cell biology
  • Mechanotransduction in hair cells

Background:

  • Acoustic overstimulation can lead to hearing loss and auditory cell death.
  • Cellular mechanisms underlying acoustic trauma remain incompletely understood.
  • Previous research indicated increased intracellular calcium during acoustic stimulation.

Purpose of the Study:

  • To develop and characterize an in vitro model for studying acoustic trauma.
  • To investigate the effects of controlled mechanical overstimulation on isolated cochlear hair cells.
  • To analyze cellular responses, including mechanical vibrations and stiffness changes, to acoustic stimuli.

Main Methods:

  • Isolated outer hair cells from guinea pig cochlea were used.
  • A micropipette delivered controlled pressure jets to mimic sound stimulation.

Related Experiment Videos

  • A second micropipette with a pressure transducer measured localized pressure.
  • A computer-controlled setup calibrated the stimulation parameters.

    • Main Results:

    • Peak pressure generated was approximately 325 Pa (144 dB) at 140 Hz.
    • Pressure jets induced significant mechanical vibrations in hair cell bodies.
    • Stimulation led to changes in cellular vibration modes, suggesting altered stiffness.
    • Most isolated hair cells demonstrated resistance to high-intensity mechanical stimulation.

    Conclusions:

    • The in vitro model effectively simulates acoustic overstimulation at the cellular level.
    • Mechanical stimulation alters hair cell mechanical properties, potentially impacting function.
    • Outer hair cells exhibit notable resilience to intense mechanical stress, warranting further investigation into protective mechanisms.