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Biochemical and genetic characterization of three molybdenum cofactor hydroxylases in Arabidopsis thaliana
T Hoff1, G I Frandsen, A Rocher
1Department of Plant Physiology, University of Copenhagen, Oester Farimagsgade 2A, 1353 Copenhagen K, Denmark. hoff@biobase.dk
Biochimica Et Biophysica Acta
|July 10, 1998
Abstract:
Aldehyde oxidases and xanthine dehydrogenases/oxidases belong to the molybdenum cofactor dependent hydroxylase class of enzymes. Zymograms show that Arabidopsis thaliana has at least three different aldehyde oxidases and one xanthine oxidase. Three different cDNA clones encoding putative aldehyde oxidases (AtAO1, 2, 3) were isolated. An aldehyde oxidase is the last step in abscisic acid (ABA) biosynthesis. AtAO1 is mainly expressed in seeds and roots which might reflect that it is involved in ABA biosynthesis.