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Related Experiment Videos

Modulation of interleukin production by ascorbic acid

J Schwager1, J Schulze

  • 1Centre de Recherche en Nutrition Animale, Société Chimique Roche S.A. Village-Neuf, France. Joseph.Schwager@Roche.com

Veterinary Immunology and Immunopathology
|July 10, 1998
PubMed
Summary

Dietary ascorbate (vitamin C) impacts pig immune cells. Lower ascorbate levels reduced lymphocyte proliferation and altered interleukin production, suggesting a role in immune homeostasis.

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Area of Science:

  • Immunology
  • Nutritional Science
  • Cell Biology

Background:

  • Ascorbate (vitamin C) is essential for numerous physiological processes.
  • Its role in immune cell function, particularly in conditions of deficiency, requires further investigation.
  • Peripheral blood mononuclear cells (PBMC) are key components of the immune system.

Purpose of the Study:

  • To investigate the influence of ascorbate levels on PBMC function in pigs with hereditary ascorbate deficiency.
  • To determine the effects of ascorbate depletion and supplementation on lymphocyte subsets and mitogen-induced proliferation.
  • To analyze the impact of ascorbate on the production of Interleukin-2 (IL-2) and Interleukin-6 (IL-6).

Main Methods:

  • Pigs with hereditary ascorbate deficiency were subjected to dietary ascorbate depletion or supplementation for up to 5 weeks.

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  • Flow cytometry was used to analyze B and T lymphocyte subsets.
  • PBMC proliferation was assessed using mitogen stimulation assays.
  • IL-2 and IL-6 production were measured via bioassays and in vitro culture.

    • Main Results:

    • Lymphocyte subsets showed only marginal and transient differences between groups.
    • PBMC proliferative responses to mitogens were significantly lower in ascorbate-depleted pigs compared to supplemented pigs.
    • Ascorbate depletion affected IL-2 and IL-6 production, with IL-2 accumulating in cultures instead of being consumed.
    • Cellular ascorbate appears to influence IL-2 production and secretion of IL-6.

    Conclusions:

    • Dietary ascorbate levels significantly impact PBMC proliferation and cytokine production in pigs.
    • Ascorbate influences immune homeostasis, potentially through reactive oxygen intermediate (ROI)-dependent regulation of interleukin gene expression via NF-kappa B.
    • These findings highlight the importance of adequate ascorbate levels for optimal immune cell function.