Validation procedures of sedimentation field-flow fractionation techniques for biological applications

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Updated: Oct 29, 2026

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
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Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Published on: October 9, 2013

E Assidjo1, T Chianéa, M F Dreyfuss

  • 1Laboratoire de Chimie Analytique et de Bromatologie, Université de Limoges, Faculté de Pharmacie, France.

Journal of Chromatography. B, Biomedical Sciences and Applications
|July 10, 1998
PubMed

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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...