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Variable gene segment-specific N-insertions at the signal joint of T-cell receptor Vbeta-Dbeta recombinations
Y Kanari1, R Nakagawa, H Arakawa
1Department of Biophysics, Graduate School of Science, Kyoto University, Japan.
The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vbeta-Dbeta recombinations. N-insertions were detected at signal joints of Vbeta2, Vbeta3, Vbeta10, Vbeta18 and Vbeta14 but not in Vbeta8 and Vbeta7. These data show that the N-insertion at signal joints is dependent on the Vbeta locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.
The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vbeta-Dbeta recombinations. N-insertions were detected at signal joints of Vbeta2, Vbeta3, Vbeta10, Vbeta18 and Vbeta14 but not in Vbeta8 and Vbeta7. These data show that the N-insertion at signal joints is dependent on the Vbeta locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.