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Alkaline phosphatase activity is expressed in murine splenic B-lymphocytes sensitized in vivo with Tetanus toxoid
1Department of Biochemistry, School of Life Sciences, University of Hyderabad, India.
Immunology Letters
|July 10, 1998
Abstract:
Alkaline phosphatase (APase) activity and proliferative response to Tetanus toxoid (TT) were measured in murine splenic lymphocytes immunized in vivo with TT. APase activity was enhanced in TT-stimulated B-lymphocytes concomitant with an increase in the proliferative response in a dose-dependent manner. Cytochemical staining for APase using beta-naphthyl phosphate also showed an increase in APase positive cells in TT-stimulated lymphocyte population. The results suggest that membrane APase expression is a physiological phenomenon occurring in antigen-stimulated B-lymphocytes.