p53 protein is a suppressor of papillomavirus DNA amplificational replication

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Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

D Lepik1, I Ilves, A Kristjuhan

  • 1Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, Tartu EE2400, Estonia.

Journal of Virology
|July 11, 1998
PubMed

Insights

The p53 protein inhibits papillomavirus DNA replication by targeting specific domains, without affecting other viral replication cycles or causing cell death. This suggests a crucial role for p53 in managing viral infections.

Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • The p53 protein is a critical tumor suppressor involved in DNA repair and apoptosis.
  • Papillomaviruses rely on host cell machinery for their replication, making them potential targets for cellular regulators.

Purpose of the Study:

  • To investigate the effect of the p53 protein on the replication of human and bovine papillomaviruses.
  • To identify the specific domains of p53 essential for its antiviral activity.

Main Methods:

  • Assessing the impact of p53 on papillomavirus DNA replication in cell cultures.
  • Mutational analysis of p53 domains to determine their role in replication suppression.

Main Results:

  • p53 effectively blocked the amplificational replication of human and bovine papillomaviruses.
  • Specific p53 domains (oligomerization, DNA-binding, RPA-binding, proline-rich) were essential for this inhibitory activity.
  • p53 did not inhibit Epstein-Barr virus replication or induce cell cycle arrest or apoptosis.

Conclusions:

  • The p53 protein possesses intrinsic activity to suppress papillomavirus amplificational replication.
  • This suppression is mediated by specific structural domains of p53 and is independent of cell cycle regulation or apoptosis.
  • p53's role in inhibiting viral replication may be significant in virus-cell interactions and the viral life cycle.

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